Bioprospecting of multi-stress tolerant Pseudomonas sp. antagonistic to Rhizoctonia solani for enhanced wheat growth promotion.

Abstract

Salt affected cotton rhizospheric soil was explored for multi-stress resistance microbes to obtain 46 rhizobacteria. Of these, seven strains strongly inhibited the growth of phytopathogenic fungus Rhizoctonia solani by virtue of antifungal compound 2,4-diacetylphloroglucinol (DAPG) production. These seven strains demonstrated an array of plant growth-promoting activities as follows: (i) production of indole-3-acetic acid, ammonia, siderophore; (ii) solubilisation of phosphate, while two isolates showed Zn solubilisation. The phenetic and 16S ribotyping revealed affiliation of all the isolates to Pseudomonas guariconensis and presence of phlD gene marker for DAPG production. Among the seven isolates, strain VDA8 showed the highest DAPG production (0.16μgml-1) in liquid synthetic medium under aerobic conditions at 28°C. Furthermore, sucrose, peptone, sodium hydrogen phosphate, ZnSO4, pH 8.0, and NaCl (1%) were observed as the best carbon, nitrogen, phosphate, trace element, pH, and salt concentration, respectively for maximum production of DAPG by strain VDA8 (3.62 ± 0.04μgml-1). The strain VDA8 was further assessed for wheat (Triticum aestivum) growth promotion by seed biopriming under laboratory (plate assay) and field condition in alkaline saline soil with pH 8.5. The field scale (324 m2) trials demonstrated 28.6% enhanced grain production compared to control demonstrating the newly isolated Pseudomonas sp. as multi-potent bioinoculant.