Abstract
The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were characterized isolated filamentous fungi by the following genera: Alternaria sp., Fusarium sp., Geotrichum sp., Gliocladium sp., Mucor sp., Paecilomyces sp. and Trichoderma sp. Extracellular lipase production was observed in 71.43% of the bacteria and 57.14% of the fungi. The bacterium that presented better promising enzymatic activity was E. aerogenes (1.54 U/ml) however between fungi there was not significant difference between the four isolates. This study indicated that microorganisms lipase producers are present in the industrial effluents, as well as these enzymes have potential of biodegradation of lipid compounds.
Highlights
The lipase enzymes are classified as hydrolases that catalyze the hydrolysis of triglycerides into diglycerides, monoglycerides, free fatty acids and glycerol (Almeida et al 2013)
Considering the existing environmental microbial diversity and the various microorganisms capable of producing lipases enzymes, it is highlighted the importance of these industrial effluents for biodegradation of lipid compounds. The aim of this present study was to isolate and identify microorganisms that produce these enzymes in industrial effluents, just like evaluation of production extracellular of the lipase and capacity of hydrolysis produced by microorganisms
The bacteria identified through the automatic system Vitek 2®, belong to five different species
Summary
The lipase enzymes are classified as hydrolases (triacylglycerol hydrolases) that catalyze the hydrolysis of triglycerides into diglycerides, monoglycerides, free fatty acids and glycerol (Almeida et al 2013). Besides the hydrolysis, they can catalyze esterification and. Many microorganisms are lipase producers as well as the main part of commercial lipases is from microbial origin (Veerabagu et al 2013). In this context, the lack of knowledge of this microbial diversity in effluents motivates to interest in the microorganisms bioprospecting, because they may show biotechnological application potential in different areas. They can be promising in technologies used in biological systems for the environment treatment with the purpose of eliminating pollutants (Peixoto et al 2008)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
