Abstract

Fucoxanthin from brown algae has potential as a promising functional food component. However, fucoxanthin is less stable under high temperature and light exposure. The purpose of this study was to analyse the effect of stevia and cacao butter in increasing the stability of Sargassum polycystum C. Agardh extract containing fucoxanthin and its application as a snack bar. Brown algae, S. polycystum, was extracted using 96% foodgrade ethanol. Antioxidant activity and stability assay were performed using the 2,2- Diphenyl-1-Picrylhydrazyl (DPPH) method. Antioxidant activity of both, extract (E) and extract + stevia (ES), was found to decrease with time. Meanwhile, cacao butter was able to maintain extract’s stability without any significant difference when compared to extract alone. A combination of extract, stevia, and cacao butter (ESC) were found to be able to maintain antioxidant stability. Formulation of a snack bar with cacao butter and 1% stevia were more preferred by the panellists when compared to the other formulations. This study reports that cacao butter and 1% stevia is able to maintain antioxidant stability and improve the economic value of S. polycystum, one of them through the formulations of the snack bar.

Highlights

  • Bioprospecting for sustainable antioxidant sources is promising opportunity since nature provides an abundant source of bioactive compounds

  • The concentration of fucoxanthin obtained in this study, measured with High-Performance Liquid Chromatography (HPLC), was 1.79 mg/g extract

  • Antioxidant activity of S. polycystum extract and individual ingredients was in descending order were as follows: ascorbic acid > rice crispy > S. polycystum extract > cacao butter > raisin > stevia powder > almond > dried coconut > rolled oats > creamer powder (Table 3)

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Summary

Introduction

Bioprospecting for sustainable antioxidant sources is promising opportunity since nature provides an abundant source of bioactive compounds. Limited application of marine resources is unfortunate as invertebrates, microalgae, and macroalgae that are usually found in these areas produce unique chemical substances with different biological activities (Montaser and Luesch, 2011; Martins et al, 2014). Macroalgae, such as Sargassum, has been used for ages in Traditional Chinese Medicine as seaweed medicine (Liu et al, 2012). Macroalgae are highly distributed and can be found in abundance They can be harvested and huge potential lies in exploring and developing them as a commercial product. It is the potential to explore and develop as commercial product, especially for coastal communities (Loureiro et al, 2015; Kim et al, 2017)

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