Abstract
xopolysaccharides are high molecular weight polymers that consist of sugar residues which widely vary in structure and function. Many micro-organisms secrete extracellular polymeric substances (EPS) during their life cycle. Azotobacter is one such bacterium that secretes EPS at suitable condition. In this study, Azotobacter were isolated from soil samples to explore the EPS production. Bacteria were identified by morphological, cultural and biochemical characteristics. The isolates KE and GE were found to be potential isolates for EPS production. Quantitative analysis by phenol sulfuric acid method showed KE containing 42 mg/ml and GE 52 mg/ml carbohydrates in the EPS. Further the functional group was also detected by using the FTIR spectrum from EPS.
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