Abstract
D-psicose exits in an extremely small amount in nature and is difficult to be chemically synthesized. Only three bacteria have been used in the biotransformation of D-psicose from D-fructose. In this paper, another bacterium which could convert D-fructose to D-psicose was isolated and identified as Rhodobacter sphaeroides. The process parameters of D-psicose production using permeabilized cells of Rhodobacter sphaeroides SK011 were optimized, including the permeabilization procedure: 0.1% (w/v) CTAB, 10 min, and reaction conditions: cell concentration, 30 g dry cell wt/L; concentration of substrate, 50 g/L; 40°C, pH 9.0; reaction time, 8 h. Under the optimized conditions, the permeabilized cells produced approximately 6.5 g/L D-psicose with a D-psicose productivity of 0.82 g·L−1·h−1. This is the first report of bioproduction of D-psicose using permeabilized cells of Rhodobacter sphaeroides.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
