Abstract
Cellulase was produced by Cellulomonas uda utilizing banana stem waste by solid state fermentation (SSF). The different parameters effects considered like particle size of substrate, pH(incubation and extraction), incubation period, temperature (incubation and extraction), media components (yeast extract and peptone) and moisture of substrate on the production of cellulase was investigated. The optimum activity of cellulase produced by Cellulomonas uda on banana waste for particle size (6.97 IU/min for 1mm), extraction pH (7.13 IU/min for pH 7), incubation pH(6.97 IU/min for pH 7), extraction temperature (7.10 IU/min for 500C), incubation temperature (7.20 IU/min for 450C), incubation period (7.20 IU/min on 3rd day), moisture content (7.12 IU/min for 100% ), peptone content ( 7.23 IU/min for 0.5 gm) and yeast extract content (7.18 IU/min for 0.30gm) was recorded. The cellulase produced by Cellulomonas uda by using banana stem waste indicates the socio-econic utilization of agricultural waste. After purification and characterization; cellulase enzyme can be used in industrial processes.
Highlights
Cellulose represents about 1.5 × 1012 tons of biomass produced by photosynthesis and is most inexhaustible raw material source for different products [1]
Effect of varying particle size on cellulase enzyme activity was considered by taking banana stem as solid substrate
Substrate collected on the sieve for example 1mm by passing through sieve of 1.4mm was considered as 1.4mm particle size.Different particle sized substrate was taken in 250 ml conical flask and solid state fermentation (SSF) was performed for 36 hrs. at 37 0 C
Summary
Cellulose represents about 1.5 × 1012 tons of biomass produced by photosynthesis and is most inexhaustible raw material source for different products [1]. Hydrolysis of lignocellulosic waste by microbial degradation and ultilization of resultant sugars (reducing) by fermentation process for production of bioproducts or biofuel will be most promising strategy for efficient utilization of this biomass [3]. Different class of Cellulases enzyme produced by a number of microorganisms helps in degradation of lignocellulosic waste through ferementation proce [4]. Media used in cellulose fermentation consists of cellulose in different degree of purity for submerged fermentation [5] and raw lignocellulosic substrate for solid state fermentation [6]. Cellulases hydrolyze cellulose (ß-1,4-D-glucan linkages) and produce as primary products glucose, cellobiose and cellooligosaccharides. There are three major types of cellulase enzymes [Cellobiohydrolase
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