Abstract
The utilization of crude waste streams and the development of novel bioprocessing routes are necessary in order to expand the industrial implementation of bio-based succinic acid production. This work focuses on the utilization of zero cost municipal organic biowaste for the production of bio-based succinic acid using the engineered yeast strain Yarrowia lipolytica PSA02004. Fermentation advances focused on pH regulation towards reduced NaOH requirements. Optimum initial carbon source concentration (100–120 g/L) in crude hydrolysates was estimated in shake flask cultures. Optimum kLα (183.2 h−1) in fed-batch cultures resulted in 42.2 g/L succinic acid with 0.38 g/g yield and 0.84 g/L/h productivity. A two-stage pH regulation strategy was employed to enhance succinic acid production efficiency and reduce NaOH requirements. The gradual reduction of pH from 6 to 5.5 resulted in 54.4 g/L succinic acid with 0.44 g/g yield and 0.82 g/L/h productivity and 43% lower NaOH consumption. The minimum selling price of succinic acid was estimated at $2.7/kg and $2.94/kg at annual production capacity of 50,000 t when the production cost of organic biowaste derived sugars were considered at $10/tTS and $110/tTS, respectively. The global warming potential and abiotic depletion potential were estimated at 2.72 kgCO2-eq/kgSA and 33.4 MJ/kgSA, respectively. Results showed that pH regulation is an important operating parameter towards the sustainable succinic acid production using municipal organic biowaste.
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