Abstract
The xylooligosaccharides (XOS, prebiotics) production from xylan extracted from sugarcane bagasse (SB) biomass was accomplished using an in-house produced xylosidase-free endoxylanase from an isolate Aspergillus flavus MG-7. Delignification of SB by sodium hypochlorite, and xylan extraction by coupled sodium hydroxide and thermal treatment were optimized for various process variables based on Design of Experiments (DoE) to achieve enhanced xylan yield (96.11%, w/w). The extracted xylan was analysed by Fouriertransform infrared spectroscopy and thermogravimetric analysis to evaluate its structural morphology, purity and thermal stability. Xylan hydrolysate obtained by endoxylanase was quantified for xylobiose (69.57%, w/w), xylotriose (13.17%) and xylotetrose (8.377%), and XOS were examined by 1H NMR. Growth-promoting potential of XOS was established for a probiotic Lactobacillus plantarum M-13 isolated from kalarei. XOS exhibited excellent bioactivity potential in terms of high positive prebiotic activity score, significant antioxidant activity and antibacterial activity. Efficient XOS production promises value-addition for biorefining of SB biomass.
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