Bioprocess development for bacterial cellulose biosynthesis by novel Lactiplantibacillus plantarum isolate along with characterization and antimicrobial assessment of fabricated membrane

Ahmed K Saleh,Waleed K El-Zawawy,Yasser R Abdel-Fattah,Hamada El-Gendi,Nadia A Soliman

doi:10.1038/s41598-022-06117-7

Ahmed K Saleh, Waleed K El-Zawawy + Show 3 more

Open Access

https://doi.org/10.1038/s41598-022-06117-7

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Abstract

Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications. Its use is limited by the capacity of bacterial production strains and cost of the medium. Mining for novel organisms with well-optimized growth conditions will be important for the adoption of BC. In this study, a novel BC-producing strain was isolated from rotten fruit samples and identified as Lactiplantibacillus plantarum from 16S rRNA sequencing. Culture conditions were optimized for supporting maximal BC production using one variable at a time, Plackett–Burman design, and Box Behnken design approaches. Results indicated that a modified Yamanaka medium supported the highest BC yield (2.7 g/l), and that yeast extract, MgSO4, and pH were the most significant variables influencing BC production. After optimizing the levels of these variables through Box Behnken design, BC yield was increased to 4.51 g/l. The drug delivery capacity of the produced BC membrane was evaluated through fabrication with sodium alginate and gentamycin antibiotic at four different concentrations. All membranes (normal and fabricated) were characterized by scanning electron microscope, Fourier transform-infrared spectroscopy, X-ray diffraction, and mechanical properties. The antimicrobial activity of prepared composites was evaluated by using six human pathogens and revealed potent antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus mutans, with no detected activity against Pseudomonas aeruginosa and Candida albicans.

Highlights

Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications
After 7 days of incubation, the flask with rotten apple was reported as a positive result for BC production as the formation of a creamy white gelatinous mat-like structure (BC pellicle) at the air–liquid interface was observed that was not destroyed by vortexing or high-speed centrifugation
The AS.6 strain 16S rRNA gene sequence was deposited in the GenBank under the accession number (MW857479.1), under the name of Lactiplantibacillus plantarum strain AS.6 (L. plantarum AS.6)

Summary

Introduction

Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications. SED involves a multistep approach, wherein a vast number of variables are simultaneously studied in the same experiment using screening designs such as Plackett–Burman design (PBD) After this screening stage, the precise role of each variable on process yield and the nature of variable interactions can be determined through optimization designs, such as Box Behnken design (BBD), that determine the optimum level for the most important variables. The work novelty can be summarized in two points: Firstly, using of probiotic isolate for BC production namely L Plantarum this is considered the first reported BC production by this species and at the same moment following up different strategies for maximizing the production using a low cost medium. The antimicrobial activity of the resulting BC membrane fabricated to SA and GM was evaluated

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