Abstract
Some microalgae are producers of high-value phytochemicals of interest for industrial applications. Cultivation of Dictyosphaerium chlorelloides microalgae yielded about 1g/L of a viscous exobiopolymer with a high molecular weight (Mp=9.6×105g/mol). Its proteoglycan character was confirmed by the presence of proteins and carbohydrates. Dominant sugars of the biopolymer were galactose and its methyl-derivatives (~42wt%), rhamnose (18wt%), mannose (14wt%) and unidentified methyl-hexoses (12wt%). Besides, glucose and 3-O-methyl derivative (8wt%), xylose and 4-O-methyl-derivative (4wt%), and arabinose (2wt%) were found. Methylation analysis indicated a complex chemical structure. Immunobiological studies revealed the effective anti-proliferative capacity of the biopolymer in vitro in both cancer and non-cancer cell lines. Based on in vitro exposition using a reconstructed 3-D tissue model, a good bioavailability and a low toxicity of the biopolymer have been found. Exposition of RAW 264.7 macrophages to the biopolymer resulted into significant immunostimulation of pro- and anti-inflammatory cytokines, and decreased free radical cell release.
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