Biopolymer Hydrogel Scaffolds Containing Doxorubicin as A Localized Drug Delivery System for Inhibiting Lung Cancer Cell Proliferation.

Chuda Chittasupho,Jakrapong Angklomklew,Wongwit Senavongse,Pensak Jantrawut,Warintorn Ruksiriwanich,Thanu Thongnopkoon

doi:10.3390/polym13203580

Chuda Chittasupho, Jakrapong Angklomklew + Show 4 more

Open Access

https://doi.org/10.3390/polym13203580

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Journal: Polymers	Publication Date: Oct 17, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Chiang Mai University, Srinakharinwirot University

Abstract

A hydrogel scaffold is a localized drug delivery system that can maintain the therapeutic level of drug concentration at the tumor site. In this study, the biopolymer hydrogel scaffold encapsulating doxorubicin was fabricated from gelatin, sodium carboxymethyl cellulose, and gelatin/sodium carboxymethyl cellulose mixture using a lyophilization technique. The effects of a crosslinker on scaffold morphology and pore size were determined using scanning electron microscopy. The encapsulation efficiency and the release profile of doxorubicin from the hydrogel scaffolds were determined using UV-Vis spectrophotometry. The anti-proliferative effect of the scaffolds against the lung cancer cell line was investigated using an MTT assay. The results showed that scaffolds made from different types of natural polymer had different pore configurations and pore sizes. All scaffolds had high encapsulation efficiency and drug-controlled release profiles. The viability and proliferation of A549 cells, treated with gelatin, gelatin/SCMC, and SCMC scaffolds containing doxorubicin significantly decreased compared with control. These hydrogel scaffolds might provide a promising approach for developing a superior localized drug delivery system to kill lung cancer cells.

Highlights

IntroductionLung cancer is the leading cause of death in men and women, responsible for almost
Lung cancer is the leading cause of death in men and women, responsible for almost25% of all cancer deaths [1]
Hydrogel scaffolds were fabricated by modulating the composition of the polymer and the crosslinker to provide properties according to specific applications

Summary

Introduction

Lung cancer is the leading cause of death in men and women, responsible for almost. 25% of all cancer deaths [1]. Chemotherapy is the first-line therapy for small-cell lung cancer (SCLC) pre-operation and post-operation, and stage IV non-small cell lung cancer (NSCLC) [2]. Chemotherapy is used as an adjuvant chemotherapy to kill remaining cancer cells and to reduce the chance of recurrence. Adjuvant chemotherapy shrinks the tumor before surgery and reduces the spread of cancer, making surgery less invasive and more effective. Chemotherapy is commonly administered to patients intravenously at the maximum tolerable doses, which may cause severe toxicity in healthy tissues [3]. Patient survival rates from chemotherapy treatments are high, drug resistance and drug-related toxicity severely limit clinical outcomes [4].

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