Biophysical study of DC electric field induced stable formation of albumin-gold nanoparticles corona and curcumin binding

Abstract

Targeted drug delivery (TDD) is a method of delivering optimum concentrations of pharmaceutical substances in the tissue to achieve the desired therapeutic effect. Hence, TDD systems are considered as an emerging strategy to deliver the drug at the specific site of the tissues/cells. The nanoparticle-protein corona as a drug delivery vehicle has demonstrated immense benefits including potential theragnostic, improved pharmacodynamics and targeted drug delivery. In the present investigation, efforts have been to establish stable and functionalized Bovine serum albumin–gold nanoparticle (BSA-GNP) corona (conjugates) using a Direct Current (DC) electric field. With the application of DC electric field (DEF) across the BSA-GNP solution, the formation of BSA-GNP corona/conjugate takes place which was characterized using various biophysical techniques such a Dynamic Light Scattering (DLS), UV Visible spectroscopy, Fluorescence spectroscopy, electrophoresis, etc. Furthermore, the DEF engineered BSA-GNP corona was loaded/interacted with curcumin (CUR). The size of the BSA-GNP corona was increased with increasing DC voltage (5–30 V) at constant concentration of BSA. The strong and stable binding of curcumin with BSA-GNP corona was revealed by the techniques used in the investigation; however, binding affinity of CUR was decreased for 30 V DEF exposed BSA-GNP conjugate. The biocompatible experimental data confirms the nontoxic nature of BSA-GNP corona. This investigation adds a new and novel physical method for the preparation of protein-nanoparticle corona for various applications including drug delivery.