Abstract
For the first time Helix lucorum hemocyanin (HlH) has been feruloylated. Two HlH conjugates with 40- and 120- ferulic acid residues were prepared, denoted as FA-HlH-1 and FA-HlH-2. Expectedly, the feruloylation of HlH induced a rearrangement of the protein molecule, a decrease in the ?-helical structure at the expense of ß-structures was observed. Besides, the FA-HlH conjugates were more prone to aggregation, which is probably due to the stabilization of the partially unfolded protein molecules by non-covalent bonding. Interestingly, the thermal stability of HlH was not affected by the modification. The native and feruloylated HlH were not toxic to normal fibroblasts (BJ cells). We observed a decrease in cell viability of breast cancer MCF-7 cells to about 66% after a 48h exposure to 70 µg/well of FA-HlH-2.
Highlights
Ferulic acid (FA) is a naturally occurring antioxidant, radical scavenging and photoprotective agent with huge therapeutic potential
The FA-Helix lucorum hemocyanin (HlH) conjugates were more prone to aggregation, which is probably due to the stabilization of the partially unfolded protein molecules by non-covalent bonding
We observed a decrease in cell viability of breast cancer MCF-7 cells to about 66% after a 48h exposure to 70 μg/well of FA-HlH-2
Summary
Ferulic acid (FA) is a naturally occurring antioxidant, radical scavenging and photoprotective agent with huge therapeutic potential. It has been reported that FA has strong hepatoprotective activity.[1] Much attention has been paid in the literature on the beneficial role of FA in the treatment and prevention of Alzheimer’s disease, a serious neurodegenerative disorder.[2] In addition, FA inhibits melanin production, can be involved in formulations for skin depigmentation.[3] Interestingly, graft copolymers containing ferulic and hyaluronic acid exhibit wound healing properties and can be involved in medicines for the treatment of chronic and acute wounds.[4] FA is considered to have potential as an anti-inflammatory drug for curing endometritis. Liu et al have shown that daily supplements of a sodium ferulate increased the relative abundance of Lactobacillus and Parabacteroides in mice model of transverse aortic construction and the changes in the gut microbiota resulted in an improved cardiac function in the tested animals.[9]
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