Abstract
This study utilized the FTIR technique to investigate and assess the herbicide 2, 4-Dichlorophenoxyacetic acid-induced blood serum toxicity. The study was conducted on 15 albino Wistar rats, divided into two groups: a control group (5 rats) and an intoxicated group (10 rats). Serum samples were investigated using the FTIR technique, and the obtained spectra from both groups were analyzed. Our results indicated a reduction in glucose, lipid, and protein content and remarkable changes in the secondary structure of protein in response to herbicide toxicity. A rise in the DNA content was also noticed. Our findings prove the FTIR technique's capability to detect biochemical changes in biological samples due to toxicity.
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