Abstract
Recent work has introduced a new fluorescent voltage sensor, ASAP1, which can monitor rapid trains of action potentials in cultured neurons. ASAP1 is based on the Gallus gallus voltage sensitive phosphatase (GgVSP) with a circularly permuted GFP placed in the S3-S4 linker. However, many of the biophysical details of this indicator remain unknown. In this work, we study the biophysical properties of ASAP1. Expressing this molecule in Xenopus oocytes and recording fluorescence simultaneously with gating current using the cut-open voltage clamp technique, our studies show that the gating currents of ASAP1 are significantly faster than CiVSP.
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