Abstract
Bionanotechnology is a relatively new term that implies the use of some biological material or organisms in order to prepare nanosystems or nanoparticles. This work presents the preparation and bactericide application of a sustainable nanometric system (silver nanoparticles) using a waste biological support (bovine bone powder). This system was prepared by the method of metallic salt reduction, using NaBH4 as reducing agent and AgNO3 as metallic salt. Two silver contents were analyzed, 1% and 5% weight. The latter was found to be more efficient than the former. Transmission electronic microscopy shows an average size of 10.5 ± 3.3 nm and quasi-sphere morphology. The antimicrobial assay shows that a 5% weight content of silver had a bactericide effect for Escherichia coli at 46.8 min of exposure. The minimum inhibitory concentration (MIC) of silver nanoparticles supported on bovine bone powder for Escherichia coli was 7.5 µg/mL. The biocomposite exhibits a specific antibacterial kinetics constant (k) of 0.1128 min−1 and decimal reduction time (DRT) of 20.39 min for Escherichia coli. Thus, it was concluded that a biocomposite was prepared with a biodegradable, waste, and low-cost support, under mild conditions (room temperature and atmospheric pressure) and using water as solvent.
Highlights
Nanometric systems have been the subject of study of a large number of articles in the last decades.This is because nanostructured systems have better chemical, mechanical, and catalytic properties than bulk materials and can be applied in various technologies
The objective of this work was to look for a sustainable bactericide system, environmentally friendly, with a natural support, that could be synthesized under room temperature, atmospheric pressure, and using water as a solvent
Silver nanoparticles were synthesized on bovine powder and the resulting system was tested in the elimination of a Gram negative bacterium such as Escherichia coli
Summary
Nanometric systems have been the subject of study of a large number of articles in the last decades This is because nanostructured systems have better chemical, mechanical, and catalytic properties than bulk materials and can be applied in various technologies. Silver nanoparticles were synthesized on bovine powder and the resulting system was tested in the elimination of a Gram negative bacterium such as Escherichia coli. This organism was proposed because it is one of the most difficult to treat, being the cause of severe diarrhea due to consumption of water contaminated with coliforms. This type of bacteria has become multi-resistant to antibiotics, making its treatment rather difficult and is an important cause of death in places where people do not have access to drinking water [4,9,10,11,12,13,14,15,16,17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
