Abstract
A simple and short-term in vitro assay system has been adapted for quantitative evaluation of the rate of phagocytosis of amoebocytes obtained from hemolymph of the pulmonate gastropod Biomphalaria glabrata. The variation in the rate of phagocytosis exhibited by amoebocyte monolayers prepared from individual snails is reduced by utilizing amoebocytes from pooled hemolymph from snails of similar sizes. The study demonstrates that the rate of phagocytosis depends on incubation time, temperature, and pH. Substantial inhibition of phagocytosis is exerted by 2-deoxy- d-glucose and 2-iodoacetamide but not by potassium cyanide, indicating that energy consumed during phagocytosis is generated by the glycolysis pathway.
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