Biomolecules and Biomarkers Used in Diagnosis of Alcohol Drinking and in Monitoring Therapeutic Interventions.

Radu M Nanau,Manuela G Neuman

doi:10.3390/biom5031339

Abstract

Background: The quantitative, measurable detection of drinking is important for the successful treatment of alcohol misuse in transplantation of patients with alcohol disorders, people living with human immunodeficiency virus that need to adhere to medication, and special occupational hazard offenders, many of whom continually deny drinking. Their initial misconduct usually leads to medical problems associated with drinking, impulsive social behavior, and drunk driving. The accurate identification of alcohol consumption via biochemical tests contributes significantly to the monitoring of drinking behavior. Methods: A systematic review of the current methods used to measure biomarkers of alcohol consumption was conducted using PubMed and Google Scholar databases (2010–2015). The names of the tests have been identified. The methods and publications that correlate between the social instruments and the biochemical tests were further investigated. There is a clear need for assays standardization to ensure the use of these biochemical tests as routine biomarkers. Findings: Alcohol ingestion can be measured using a breath test. Because alcohol is rapidly eliminated from the circulation, the time for detection by this analysis is in the range of hours. Alcohol consumption can alternatively be detected by direct measurement of ethanol concentration in blood or urine. Several markers have been proposed to extend the interval and sensitivities of detection, including ethyl glucuronide and ethyl sulfate in urine, phosphatidylethanol in blood, and ethyl glucuronide and fatty acid ethyl esters in hair, among others. Moreover, there is a need to correlate the indirect biomarker carbohydrate deficient transferrin, which reflects longer lasting consumption of higher amounts of alcohol, with serum γ-glutamyl transpeptidase, another long term indirect biomarker that is routinely used and standardized in laboratory medicine.

Highlights

Alcoholism and alcohol misuse encompass a spectrum of injury that affects all the organs and tissues of the body [1,2]
The ALDH2*2 and the ADH2*2 alleles, as well as the c2 allele of the cytochrome p450 2E1 (CYP2E1) gene are unique to Orientals
This study shows that each method has its specific timeframe during which it is useful, and multiple methods should ideally be used together in order to identify immediate, short-term or long-term alcohol consumption

Summary

Introduction

Alcoholism and alcohol misuse encompass a spectrum of injury that affects all the organs and tissues of the body [1,2]. The ALDH2*2 and the ADH2*2 alleles, as well as the c2 allele of the cytochrome p450 2E1 (CYP2E1) gene are unique to Orientals This prompted Sun et al [7] to analyze their involvement in the drinking behavior in 322 middle-aged. The quantitative, measurable detection of drinking is important for the successful treatment of alcohol misuse in transplantation of patients with alcohol disorders, people living with human immunodeficiency virus that need to adhere to medication, and special occupational hazard offenders, many of whom continually deny drinking. Their initial misconduct usually leads to medical problems associated with drinking, impulsive social behavior, and drunk driving. There is a clear need for assays standardization to ensure the use of these biochemical tests as routine biomarkers

Methods

Results

Discussion

Conclusion