Abstract
Abstract Two new HPLC packing materials, HydrophaseTM HP-SAX and HP-SCX, based upon a macroporous hydrophilic polymer, have been developed for biomolecule separations. Hydrophase HP-SAX was developed by attaching quaternary nitrogen groups onto the surface of the base polymer for anion-exchange separations. HP-SCX was formed by attaching sulfonyl groups to the base polymer for cation-exchange chromatographic separations. Both HP-SAX and HP-SCX have high efficiency/theoretical plate ratings, broad pH stability range, tolerate high pressures (up to 2250 psi), and can be operated at high flow rates. Data are presented which illustrate the resolution characteristics of the resins at highflow rates, up to 3 mL/min. Additional experiments demonstrate protein/enzyme loading, separation and recovery. The resolution and recovery analyses using these polymeric materials reveal the versatility and reliability of HP-SAX and HP-SCX columns.
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