Abstract

Summary The introduction of biomolecular methods into routine veterinary diagnostic work flow for the poultry industry dramatically changed and improved the overall diagnostic possibilities, especially with regards to fast detection of viruses. The biomolecular methods based on amplification techniques such as Polymerase Chain Reaction and its derivatives and on subsequent or real-time detection, were extensively implemented in poultry veterinary diagnostics in the last three decades. Today all essential components, such as PCR thermal cycler, software, detection kits and on-site training, needed to establish an accredited lab routine for modern molecular methods, e.g. in an integrated poultry producing company, are available. Other biomolecular methods, such as those based on isothermal amplification, feature significant advantages over Polymerase Chain Reaction and its derivates and may be of importance in the future, but were not recognized by routine veterinary diagnosticians adequately, yet.

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