Biomolecular linear motors confined to move upon micro-patterns on glass

Abstract

Biomolecular linear motor proteins—kinesin and microtubules—are finely patterned on a conventional, flat glass substrate using a Parylene lift-off process. These patterns are useful for single molecule analysis and biohybrid transportation. A patterning width of 2 µm has been achieved with minimal non-specific binding of the proteins. A conventional gliding assay was realized using this glass substrate and behaviors of the single proteins were analyzed.