Abstract
Lavandula Stoechas L. is widely known for its pharmacological properties. This study was performed to identify its biomolecules, which are responsible for enhancement of memory. L. stoechas aqueous extract was first purified by liquid column chromatography. The purified fractions were analyzed for in vitro anti-cholinesterase activity. The fraction that produced the best anti-cholinesterase activity was named an active fraction of L. stoechas (AfL.s). This was then subjected to GC–MS for identifications of biomolecules present in it. GC–MS indicated the presence of phenethylamine and α-tocopherol in AfL.s. Different doses of AfL.s were orally administered (for seven days) to scopolamine-induced hyper-amnesic albino mice and then behavioral studies were performed on mice for two days. After that, animals were sacrificed and their brains were isolated to perform the biochemical assay. Results of behavioral studies indicated that AfL.s improved the inflexion ratio in mice, which indicated improvement in retention behavior. Similarly, AfL.s significantly (p < 0.001) reduced acetylcholinesterase and malondialdehyde contents of mice brain, but on the other hand, it improved the level of choline acetyltransferase, catalase, superoxide dismutase, and glutathione. It was found that that high doses of AfL.s (≥400 mg/Kg/p.o.) produced hyper-activity, hyperstimulation, ataxia, seizures, and ultimate death in mice. Its LD50 was calculated as 325 mg/Kg/p.o. The study concludes that α-tocopherol and phenethylamine (a primary amine) present in L. stoechas enhance memory in animal models.
Highlights
Lavandula stoechas L. (Lamiaceae) is an aromatic and medicinal plant of the Mediterranean region
L. stoechas aqueous extract was fractionated further by column chromatograp total, 55 fractions were obtained by using different solvent systems
This effect would be due to the action of phenethylamine on trace amine-associated receptors (TAARs), which are abundantly present in the brain, pituitary glands, kidney, liver, and stomach [26]
Summary
Lavandula stoechas L. (Lamiaceae) is an aromatic and medicinal plant of the Mediterranean region. (Lamiaceae) is an aromatic and medicinal plant of the Mediterranean region It was the most popular folk remedy for the management of digestive disorders, kidney disease, diabetes mellitus, hyperlipidemia, cough, asthma, headache, and flu-like symptoms [1,2]. It is known as “broom of the brain” due to its extensive use in the treatment of migraines, epilepsy, and memory related disorders [3,4]. Linalool present in L. stoechas has a sedative effect [7] and it acts on different brain receptors to modify behavioral patterns, along with creating a sense of calmness and wellbeing. Keeping in mind the traditional uses of L. stoechas, as a neurotonic and memory enhancer, as part of our studies on the methanolic extract of L. stoechas [15], our current study was designed to report the active ingredient of L. stoechas aqueous extract responsible for nootropic activity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
