Abstract
We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio (m/z) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest m/z clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law (r2 > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.
Highlights
Matrix-assisted laser desorption/ionization (MALDI), an important and valuable ionization technique used in mass spectrometry [1,2], has been widely applied for the analysis of large biomolecules such as proteins and peptides [3]
We have demonstrated that our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector can be used to quantitatively detect very large cluster ions
Compared to the previous literature [8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,55,56,63,64,65], we present the fifirst report on the evaluation of large biomolecular ion cluster distributions from the monomer to mega Dalton region using the MMALDI iion ttrraapp mmaassss ssppeeccttrroommeter wwiith aa cchhaarrggee ddeetteeccttoorr
Summary
Matrix-assisted laser desorption/ionization (MALDI), an important and valuable ionization technique used in mass spectrometry [1,2], has been widely applied for the analysis of large biomolecules such as proteins and peptides [3]. MALDI has the advantages of producing high mass ions so that the mass spectra are more suitable for biomolecule analysis [4]. Photochemical ionization [8,9,10,11,12,13,14,15,16] and cluster ionization [17,18,19,20,21,22,23] are the two major hypotheses to explain many of the experimental results in MALDI. All of the analyte ions have to come from collision
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