Abstract
Microbial Induced Calcite Precipitation (MICP) is a biochemical process widely found in nature, also known as microbial mineralization. This paper investigates whether this process can help promote the intelligent reinforcement and repair of underground projects such as mines and tunnels. We selected Bacillus sphaericus and Bacillus mucilaginosus as the research objects. The former has an outstanding urease production ability, and the latter can secrete carbonic anhydrase in vitro. Bacillus mucilaginosus was introduced into the culture solution of Bacillus sphaericus in the most suitable culture environment, and the changes of mineralization rate and mineralization yield of Bacillus sphaericus were observed and analyzed. The results revealed that, to maintain the highest growth rate of Bacillus sphaericus, the optimal pH value was between 7 and 8, the optimal urea concentration was 0.5 mol/L, the optimal Ca2+ concentration was 0.6 mol/L, and the optimal Luria‐Bertani (LB) culture concentration was 20 g/L. The amount of biomineralized calcium carbonate precipitated in the double bacteria solution can reach 1.89 times the amount of the precipitation in the Bacillus sphaericus solution under the same conditions. It concludes that the introduction of Bacillus mucilaginosus can effectively increase the mineralization yield of Bacillus sphaericus without affecting the mineralized products.
Highlights
Mineralization is a general term for the process of converting organic compounds into inorganic compounds in soil under the action of soil microorganisms. e mineralization studied in this paper is the ability of Bacillus sphaericus to produce calcium carbonate
Bacillus sphaericus can secrete urease, and the latter is capable of decomposing urea to form carbonate. e generated carbonate is combined with calcium ions in the environment to form calcium carbonate
This paper studies the biomineralization performance of Bacillus sphaericus under the action of carbonic anhydrase, based on the biochemical theory of microbial mineralization and diagenesis. is work provides a theoretical basis for the engineering application of microbial natural mineralization and water control
Summary
Mineralization is a general term for the process of converting organic compounds into inorganic compounds in soil under the action of soil microorganisms. e mineralization studied in this paper is the ability of Bacillus sphaericus to produce calcium carbonate. E mineralization studied in this paper is the ability of Bacillus sphaericus to produce calcium carbonate.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
