Abstract
The present study illustrates the influence of microaerophilic condition on periodic discontinuous batch reactor (PDBR) operation in treating azo dye containing wastewater. The process performance was evaluated with the function of various dye load operations (50–750mg/l) by keeping the organic load (1.6kg COD/m3-day) constant. Initially, lower dye operation (50mg dye/l) resulted in higher dye [45mg dye/l (90%)] and COD [SDR: 1.29kg COD/m3-day (92%)] removal efficiencies. Higher dye load operation (750mg dye/l) also showed non-inhibitory performance with respect to dye [600mg dye/l (80%)] and COD [1.25kg COD/m3-day (80%)] removal efficiencies. Increment in dye load showed increment in azo reductase and dehydrogenase activities (39.6U; 4.96μg/ml; 750mg/l). UV–Vis spectroscopy (200–800nm), FTIR and 1H NMR studies revealed the disappearance of azo bond (–NN–). First derivative cyclic voltammogram supported the involvement of various membrane bound redox shuttlers, viz., cytochrome-C, cytochrome-bc1 and flavoproteins (FAD (H)).
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