Biomineralization behavior and mechanism of microbial-mediated removal of arsenate from water

Abstract

The microbial-mediated removal of arsenate by biomineralization received much attention, but the molecular mechanism of Arsenic (As) removal by mixed microbial populations remains to be elucidated. In this study, a process for the arsenate treatment using sulfate-reducing bacteria (SRB) containing sludge was constructed, and the performance of As removal was investigated at different molar ratios of AsO43- to SO42-. It was found that biomineralization mediated by SRB could achieve the simultaneous removal of arsenate and sulfate from wastewater but only occurred when microbial metabolic processes were involved. The reducing ability of the microorganisms for the sulfate and arsenate was equivalent, so the precipitates produced at the molar ratio of AsO43- to SO42−of 2:3 were most significant. X-ray absorption fine structure (XAFS) spectroscopy was the first time used to determine the molecular structure of the precipitates which were confirmed to be orpiment (As2S3). Combined with the metagenomics analysis, the microbial metabolism mechanism of simultaneous removal of sulfate and arsenate by the mixed microbial population containing SRB was revealed, that is, the sulfate and As(V) were reduced by microbial enzymes to produce S2- and As(III) to further form As2S3 precipitates. This research provided a reference and theoretical foundation for the simultaneous removal of sulfate and arsenic mediated by SRB-containing sludge in wastewater treatment.