Biomimetic Synthesis of Silver Nanoparticles Using Bhimkol (Musa balbisiana) Peel Extract as Biological Waste: Its Antibacterial Activity and Role of Ripen Stage of the Peel

Abstract

Background: Utilization of plant extracts and agricultural waste has a great impact for the synthesis of AgNPs. Banana peels are such important agro waste which attracted us to use for the synthesis of silver nanoparticles. The biochemicals present in it have attracted us to use such banana peels. Methods: Thus, we report herein a cost-effective and environment-friendly synthesis of silver nanoparticles using Bhimkal (Musa balbisiana) peel aqueous extract as biological waste. About 5 g of freshly dried peels taken in 100 mL of water were shaken and heated at 80°C for 1 hour. The filtrate from the resultant solution was stored at 4°C and used as reducing as well as stabilizing agent for the preparation of AgNPs from AgNO3. We monitored the formation of silver nanoparticles by various spectroscopic techniques. Results: All the particles are almost spherical in morphology and the diameter of the mostly monodispersed AgNPs is in the range of 30-70 nm with an average size of 44.24 nm. Among the three stages of development (unripe, ripe, and blacken), we have found the ripening stage as most efficient in the highest yielding of AgNPs because of maximum presence of phenol containing biological macromolecules. The synthesized AgNPs showed moderate antibacterial activity against both gram negative bacteria as well as gram positive bacteria. Conclusion: The advantage of our biomimetic route to silver nanoparticles lies in the fact that we utilize peels as biological waste material both for the generation and stabilization of silver nanoparticles.