Biomimetic recombinant of red blood cell membranes for improved photothermal therapy

Pengkai Wu,Tianqing Liu,Kaikai Wang,Shuai Yin,Ying Yang,Xing Jiang

doi:10.1186/s12951-021-00949-7

Abstract

BackgroundRBC membrane derived nanoparticles (NPs) represent an emerging platform with prolonged circulation capacity for the delivery of active substances. For functionalize derived RBCs NPs, various strategies, such as biomimetic rebuilding of RBCs, chemical modification or inserting ligands, have been carried out to improve their performance. However, one potential adverse effect for these methods is the structural failure of membrane proteins, consequently affecting its original immune escape function.ResultsIn this study, we reported a green technology of “disassembly-reassembly” to prepare biomimetic reconstituted RBCs membrane (rRBCs) by separating the endogenous proteins and lipids from nature RBC membrane. IR780 iodide was used as a pattern drug to verify the property and feasibility of rRBCs by constructing IR780@rRBC NPs with IR780@RBC NPs and free IR780 as controls. The results demonstrated the superiority of IR780@rRBC NPs in toxicity, stability, pharmacokinetics and pharmacodynamics compared with IR780@rRBC and free IR780.ConclusionsThe reported “disassembly-reassembly” strategy shows great potential to produce controllable and versatile rRBC membrane-inspired delivery platform, which may be used to overcome the deficiency of functionalization in cell membrane coated nanoparticles .Graphic abstract

Highlights

Red blood cell (RBC) membrane derived nanoparticles (NPs) represent an emerging platform with prolonged circulation capacity for the delivery of active substances
Preparation and characterization of IR780@RBC and IR780@reconstituted RBCs membrane (rRBCs) Several studies have reported that IR780 is tended to integrate with the hydrophobic parts of other components based on its strong hydrophobicity [23]
To characterize the reconstituted IR780@rRBC NPs, the morphology and particle size of the free IR780, IR780@ RBC and IR780@rRBC were first measured by TEM and DLS

Summary

Introduction

RBC membrane derived nanoparticles (NPs) represent an emerging platform with prolonged circulation capacity for the delivery of active substances. For functionalize derived RBCs NPs, various strategies, such as biomimetic rebuilding of RBCs, chemical modification or inserting ligands, have been carried out to improve their performance. One potential adverse effect for these methods is the structural failure of membrane proteins, affecting its original immune escape function. Wu et al J Nanobiotechnol (2021) 19:213 to improve the properties of native RBC membranes derived nanoparticles, such as deformability, stability, targeting and circulation abilities. The strategies mainly include biomimetic rebuilding of RBCs, chemical modification on the membranes surface or inserting targeted ligand-linker-lipid conjugates into the membranes [13, 14]. One potential adverse effect for these methods is the structural failure of membrane proteins, such as the self-recognition marker CD47 of preventing phagocytosis by macrophages, affecting its original immune escape function [15, 16]

Methods

Results

Conclusion