Biomimetic Properties of Force-Spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application

Kegan Mccolgan-Bannon,Muhammad Tausif,Piergiorgio Gentile,Stephen Russell,Ana Ferreira,Sarah Upson,Kenneth Dalgarno

doi:10.3390/coatings9060350

Kegan Mccolgan-Bannon, Muhammad Tausif + Show 5 more

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https://doi.org/10.3390/coatings9060350

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Journal: Coatings	Publication Date: May 28, 2019
Citations: 5	License type: CC BY 4.0

Affiliation: Newcastle University, University of Leeds

Abstract

The force-spinning process parameters (i.e., spin speed, spinneret-collector distance, and polymer concentration), optimised and characterised in previous work by this group, allowed the rapid fabrication of large quantities of high surface area poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) polymeric fibre membranes. This paper examined the potential application for force-spun PHBV fibres functionalised with type I collagen for tissue regeneration applications. PHBV fibre scaffolds provide a biologically suitable substrate to guide the regeneration of dermal tissues, however, have poor cellular adhesion properties. The grafting of collagen type-I to PHBV fibres demonstrated improved cell adhesion and growth in Neo-NHDF (neonatal human dermal fibroblasts) fibroblasts. The examination of fibre morphology, thermal properties, collagen content, and degradability was used to contrast the physicochemical properties of the PHBV and PHBV-Collagen fibres. Biodegradation models using phosphate buffered saline determined there was no appreciable change in mass over the course of 6 weeks; a Sirius Red assay was performed on degraded samples, showing no change in the quantity of collagen. Cell metabolism studies showed an increase in cell metabolism on conjugated samples after three and 7 days. In addition, in vitro cytocompatibility studies demonstrated superior cell activity and adhesion on conjugated samples over 7 days.

Highlights

Polyhydroxylalkanoates (PHAs) are a class of biocompatible, biodegradable polymers produced by microorganisms and through plant-based fermentation [1]
Surface functionalisation or blending of proteins is known to decrease the melting temperature of polymeric-based scaffolds, and this study shows that the addition of collagen reduced the melting temperature of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) [18,43]
Collagen surface functionalisation was evidenced by the presence of amide I and II bands in attenuated total reflectance (ATR)-FTIR and nitrogen contents detected by X-ray photoelectron spectroscopy (XPS) scans of about

Summary

Introduction

Polyhydroxylalkanoates (PHAs) are a class of biocompatible, biodegradable polymers produced by microorganisms and through plant-based fermentation [1]. Of this class, one of the most well-studied variants is poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV), a copolymer of polyhydroxybutyrate and polyhydroxyvalerate. Due to its favourable biological properties, PHBV has been targeted for applications in a range of tissue engineering (TE) and in vivo applications, including wound dressings, neural, dermal, osseous and cardiovascular tissues [2,3,4,5]. Nanofibres have been shown to be of value for TE by mimicking the fibrillary structure of the extracellular matrix (ECM) [6]. Electrospinning is an established technique for the preparation of nanofibres with diameters ranging from tens of nanometres to several microns [4].

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