Abstract
Organic-inorganic composite microcapsules made of sodium poly(styrenesulfonate) (PSS), poly(dimethyl diallyl ammonium chloride) (PDADMAC), and biomimetic silica were successfully fabricated via a combination of layer-by-layer self-assembly and biomimetic mineralization. The morphologies and chemical compositions of the microcapsules (denoted by (PSS/PDADMAC) 2 -SiO 2 ) were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The composite microcapsules were used as carrier for the immobilization of glucose oxidase (GOD). GOD encapsulated in (PSS/PDADMAC) 2 -SiO 2 (denoted by GOD@(PSS/PDADMAC) 2 -SiO 2 ) displayed improved thermal, pH, and operational stabilities. The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions. GOD@(PSS/PDADMAC) 2 -SiO 2 showed a 2.21-fold increase in the Michaelis constant ( K m ) compared with free GOD. The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems, and as biosensors. Glucose oxidase (GOD) was immobilized in organic-inorganic composite microcapsules using a combination of layer-by-layer assembly and biomimetic mineralization. The encapsulated GOD exhibited distinct advantages in terms of thermal, pH, and operational stabilities.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
