Abstract
AbstractThe interface between materials and cells plays a critical role in many biomedical applications. Inspired by the hierarchical architecture of collagen, most abundant structure in the extracellular matrix (ECM), a multiscale hierarchical topography is designed to mimic the collagen nano/micro hierarchical topography. It is hypothesized that the ECM topography affects osteogenesis of human mesenchymal stem cells but until now, it cannot be studied without the biochemical and mechanical influences of the ECM. The multiscale hierarchical topography is achieved by innovatively using sequentially aligned topography preparation via a silicone stretch‐oxidation‐release method and imprinting lithography. The anisotropically hierarchical topography influences stem cell morphology, orientation, and osteogenic differentiation. Intriguingly, the design resembling that of assembled collagen, exhibits the highest degree of osteogenesis. The hierarchical topotaxis effects are further exemplified by the enhanced vinculin expression, cell contractility, and more pronounced nuclear translocation of Yes‐associated protein with the collagen‐mimicking topography, indicative for enhanced osteogenesis. The developed multiscale hierarchical system provides insights into the importance of specific biological ECM‐like topography by decoupling the biochemical influence. Various diseases, cancer, osteoarthritis, and fibrosis display impaired ECM structures, and therefore this system may have a great potential for tissue engineering approaches and developing in vitro disease models.
Highlights
IntroductionThe hierarchical topotaxis effects are further exemplified by the enhanced vinculin expression, cell contractility, and more pronounced nuclear translocation of Yesassociated protein with the collagen-mimicking topography, indicative for enhanced osteogenesis
It is hypothesized that the extracellular matrix (ECM) topography affects osteogenesis of human mesenchymal stem cells but until now, it cannot be studied without the stem cells are regulated bychemical signals, biophysical cues, and cell–cell interactions.[11,12]
The developed system would provide a method to address the hypothesis that whether or not well defined hierarchically topography consisting of nano- and micropatterned structure as found in collagen bundles aid in the guidance of cells and direct the commitment of stem cells
Summary
The hierarchical topotaxis effects are further exemplified by the enhanced vinculin expression, cell contractility, and more pronounced nuclear translocation of Yesassociated protein with the collagen-mimicking topography, indicative for enhanced osteogenesis. The developed multiscale hierarchical system provides insights into the importance of specific biological ECM-like topography by decoupling the topography on the cell fate of different stem cell types,[17,18] and this can be modulated by the size and shape of surface topographical structures.[19,20,21,22,23] Aligned topographical features have been considered as a mimic of fiber-like ECM strucbiochemical influence. Cancer, osteoarthritis, and fibrosis display tures and have been used together with impaired ECM structures, and this system may have a great potential for tissue engineering approaches and developing in vitro disease models
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