Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase.

Jun-Kai Gao,Yan-Jun Jiang,Yan Chen,Zi-Jun Zhang,Shu-Feng Gao

doi:10.3390/molecules22101597

Jun-Kai Gao, Yan-Jun Jiang + Show 3 more

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https://doi.org/10.3390/molecules22101597

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Abstract

Tannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thoroughly characterized by the transmission electron microscopy (TEM), scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FT-IR) and the results showed that the Dop-TAMS possessed sufficiently large pore size and volume for the accommodation of NHase. Studying the thermal stability, storage, shaking stability, and pH stability of the free and immobilized NHase indicated that the catalytic properties of NHase@Dop-TAMS were significantly enhanced. Moreover, the NHase@Dop-TAMS exhibited good reusability. All the results demonstrated that Dop-TAMS could be used as an excellent matrix for the immobilization of NHase.

Highlights

Enzymes have a wide range of applications, including in disease diagnosis, clinical treatment, and in the food industry, due to several advantages, such as mild reaction conditions, high efficiency, and specificity [1,2]
Gao et al synthesized monodisperse core-shell magnetic organosilicanano flowers as the support for Candida antarctica lipase B (CALB) immobilization and the study results showed that the the resulting immobilized enzyme (CALB/nanoflowers) exhibited improved pH
The results showed that the Dop-templated mesoporous silica (TAMS) had great potential for the immobilization of Nitrile hydratase (NHase) in practical applications

Summary

Introduction

Enzymes have a wide range of applications, including in disease diagnosis, clinical treatment, and in the food industry, due to several advantages, such as mild reaction conditions, high efficiency, and specificity [1,2]. The application of free enzymes is still limited by low environmental adaptability, poor operational stability, and poor recyclability [3]. It is important, to overcome these drawbacks and the immobilization of enzymes on solid carriers has been demonstrated to improve their adaptability to changes in surroundings and raise their recycling rate [4,5]. The methods proposed for enzyme immobilization are distinguished as either physical or chemical. In the preparation of immobilized enzymes, it is essential to select an appropriate carrier to improve the operational stability of the biocatalyst [8,9]. Hung et al utilized 3-aminopropyltrimethoxysilane and glutaraldehyde-functionalized mesoporous silica nanoparticles (IBN-4) as the carrier of Horseradish peroxidase (HRP) and the Molecules 2017, 22, 1597; doi:10.3390/molecules22101597 www.mdpi.com/journal/molecules

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