Biomimetic Films of BLMs Based on Conductive Hybrid Films of Au NPs and Cellulose

Abstract

A new type of biomimetic films of bilayer lipid membrane (BLM) supported by conductive hybrid film of Au nanoparticles (Au NPs) and cellulose is developed. The facile preparation method of the conductive hybrid films and the relevant micro conducting mechanism under electrochemical redox environment are revealed. The regenerated cellulose film is prepared from the cellulose/N-methylmorpholine N-oxide monohydrate solution. After the cellulose film is regenerated by deionized water, Au NPs in the colloid react with the newborn cellulose film. Rectangular pieces of dry hybrid films with one ends clamped with copper foil are used as electrodes. The lipid solution of phospholipid and cholesterol with a ratio of 3:1 is brushed onto the surface of dry hybrid films. There are only reducing currents in the cyclic voltammetry responses of biomimetic films under the aqueous electrolyte solution of 0.1 mol/L KCl, 1 mmol/L K3[Fe (CN)6] and 1 mmol/L K4[Fe (CN)6]. It means that the anions of [Fe (CN)6]4- are almost impossible to be oxidized into [Fe (CN)6]3- by the positively charged surfaces of Au NPs under voltages below 0.3 V.