Biomicroplastics versus conventional microplastics: An insight on the toxicity of these polymers in dragonfly larvae

Abstract

The toxicological safety of products developed as alternative for conventional plastics (i.e., petroleum derivatives) inevitably demands conducting (eco)toxicological studies. Thus, the aim of the current study was to evaluate the biochemical toxicity of polyethylene microplastics (PE MPs) (representative of conventional MPs) and polylactic acid biomicroplastics (PLA BioMPs) in Aphylla williamsoni larvae used as experimental models. Animals subjected to short exposure to both pollutants (48 h), at environmentally relevant concentration (6 mg/L). At the end of the experiment, different toxicity biomarkers were evaluated. To assess the possible impact of pollutants on the nutritional status of the animals, the total protein, total soluble carbohydrate and triglyceride levels were determined. However, we did not observe differences between the groups, which suggests that PE MPs and PLA BioMPs did not affect the animals' energy metabolism, inducing them to a nutritional deficit. However, larvae exposed to PLA BioMPs have shown increased nitrite and lipid peroxidation levels, which supports the hypothesis that these pollutants increase oxidative stress processes in the animals evaluated, which can affect the animals' physiological homeostasis from different changes. In addition, the decrease in superoxide dismutase activity and of total thiols levels, in these same animals, is suggestive of the impact of PLA BioMPs on the antioxidant defenses, causing a REDOX imbalance, never before reported. On the other hand, decreased AChE activity was only observed in larvae exposed to PLA BioMPs, which demonstrates the anticholinergic activity of the tested polymers; the consequences of which include changes in different neurophysiological functions. Thus, the current study has helped improving the scientific knowledge about impacts caused by PLA BioMPs on freshwater ecosystems, as well as corroborated assertions about the risks posed by such biopolymers on these environments.