Abstract
In tissue culture it is necessary to use seeds of good quality and free of pathogens in order to avoid damages in the work to be carried out in the laboratory. Therefore, strict cleaning of the seeds intended for in vitro cultivation is essential. Thus, the objective of this study was to evaluate fruit biometry, germination and morphogenesis of 'Cravo' (Citrus limonia Osbeck) and Persia Lima (Citrus aurantium) seeds in vitro submitted to different concentrations of NaClO for asepsis. Fruits of 'Cravo' lemon and Lima of Persia. Subsequently, they were transported to the Biotechnology Laboratory of the Goiano Federal Institute Campus Urutaí-GO, where they performed the biometric characterization of the fruits. The seeds of these fruits were submitted to treatment with sodium hypochlorite solution in different concentrations: 0,0; 1.0; 1.5; 2.0 and 2.5% for 10 minutes, constituting then five treatments for each variety and placed in an acclimatized growth room to analyze the results. The variables evaluated were: biometry of fruits and seedlings; oxidation indexes; germination; contamination; and types of contamination. NaClO doses were compared from 95% confidence intervals. The analyzes were performed using the glm () function of software R version 3.4.1. It was verified that the aseptic treatment with NaClO for lime and lemon was promising for the morphological processes of germination and development of seedlings, as well as for the control of contaminating agents in vitro.
Highlights
A cultura de tecidos é uma ferramenta que vírus e outros agentes fitopatogênicos
Fruits of 'Cravo' lemon and Lima of Persia. They were transported to the Biotechnology Laboratory of the Goiano Federal Institute Campus Urutaí-GO, where they performed the biometric characterization of the fruits
The seeds of these fruits were submitted to treatment with sodium hypochlorite solution in different concentrations: 0,0; 1.0; 1.5; 2.0 and 2.5% for 10 minutes, constituting five treatments for each variety and placed in an acclimatized growth room to analyze the results
Summary
Germinação e morfogênese in vitro de citros após assepsia de sementes em diferentes concentrações de hipoclorito de sódio. O objetivo deste estudo foi avaliar a biometria de frutos, a germinação e morfogênese de sementes de Limão ‘Cravo’ (Citrus limonia Osbeck) e Lima da Persia (Citrus aurantium) in vitro submetidas a diferentes concentrações de NaClO para a assepsia. As sementes desses frutos foram submetidas a tratamentos com soluções de hipoclorito de sódio em diferentes concentrações: 0,0; 1,0; 1,5; 2,0 e 2,5% por 10 minutos, constituindo então cinco tratamentos para cada variedade e colocadas em sala de crescimento aclimatizada para análise dos resultados. Constatou-se que o tratamento asséptico com NaClO para lima e limão foi promissor para os processos morfológicos de germinação e desenvolvimento de plântulas, bem como para o controle de agentes contaminantes in vitro.
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