Biometric characterization and tetrazolium test in Campomanesia phaea O. Berg. Landrum seeds

Aparecida Leonir Da Silva,Victor Augusto Forti,Angelo Pedro Jacomino,Mariane Jeronimo Forte,Simone Rodrigues Da Silva

doi:10.1590/2317-1545v43240073

Aparecida Leonir Da Silva, Victor Augusto Forti + Show 3 more

Open Access

https://doi.org/10.1590/2317-1545v43240073

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Abstract

ABSTRACT: Campomanesia phaea has commercial interest because its fruits are used in the manufacture of several products, besides its high nutritional potential. Nevertheless, it has low germination potential and there is not much information in the literature. The aim of this study was to perform a biometric characterization and to establish the tetrazolium test for cambuci seeds. Seeds obtained from two collection periods were subjected to biometric evaluations (seed length, thickness, width and mass), water content, determination of the soaking curve and tetrazolium test. The soaking curve was performed with non-scarified seeds and seeds subjected to two scarification methods, mechanical and chemical. The tetrazolium test was performed soaking the seeds in a solution of 2, 3, 5 triphenyl tetrazolium chloride salt at 0.75, 1.00 and 1.50% for 4, 8 and 12 hours. After this period, the seeds were categorized as viable and non-viable. It was concluded that the cambuci seeds presented biometric differences between the two collection periods, because of the different water contents. There was no difference in the soaking curve among the three treatments tested and the solution of 0.75% for four hours in a water bath at 38 oC was considered the best for performing the tetrazolium test.

Highlights

The cambucizeiro has a stem with a peeling aspect, glabrous leaves or covered with trichomes and its fruits are disc-shaped, when ripe they have green coloring (Oliveira et al, 2020) has fruit which can be consumed in natura, in the form of jams, juices, among others, with high concentrations of ascorbic acid, minerals, fibers, flavonoids, sodium, potassium, phosphor, magnesium and calcium (Lorenzi, 2002; Vallilo et al, 2005; Bianchini et al, 2016; Tokairin et al, 2018), proven therapeutic effect (Wczassek et al, 2019), other study showed that phenolic compounds from cambuci have a potentially actions protecting mice from a high-fat, high-sucrose diet-induced glucose intolerance and adipose tissue inflammation (Donado-Pestana et al, 2015)
According to the correlation analysis (Table 2), in the first collection there was no significant correlation among the variables; in the second collection there was a significant correlation between length and width of the cambuci seeds
The different water contents in the seeds of Campomanesia phaea are determinant in their biometric parameters, regardless of the period of collection

Summary

Introduction

Landrum), a species belonging to the family Myrtaceae, native to the Brazilian Atlantic Forest (Lorenzi et al, 2006; Landrum and Kawasaki, 1997), being found throughout the Southeast region (Minas Gerais, São Paulo, Rio de Janeiro) (Oliveira et al, 2020). Genera such as Eugenia, Campomanesia, Psidium, and Myrciaria are extremely relevant and include the species of greatest economic interest (Maluf and Pisciottano-Ereio, 2005). Knowledge of the physical characteristics of the seed is important for its conservation and exploitation, addition to being a tool for the detection of genetic variability within a population of the same species (Silva et al, 2014)

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