Abstract
Multidrug-resistant A. baumannii is increasingly recognized as a significant problem in hospitals and causes high morbidity and mortality. Here, we studied the antibacterial effects of AgNPs on clinically isolated multiple drug-resistant A. baumannii, and search for the potential antibacterial mechanism. Based on the results from the colony-forming unit (CFU) method, flow cytometry (FC), and a BrdU ELISA, we conclude that AgNPs can simultaneously induce apoptosis and inhibit new DNA synthesis in bacteria in a concentration-dependent manner. This study presents the first discussion of an antibacterial effect by AgNPs in clinically isolated, multidrug-resistant A. Baumannii and provides a new strategy for the use of silver nanoparticles in the multidrug-resistant A. Baumannii clinical problem.
Highlights
Acinetobacter baumannii is an aerobic, gram-negative coccobacillus that causes nosocomial human infections, in immune-compromised individuals
Among 21 males (55.26%) and 17 females (44.74%), 13 isolates were collected from the intensive care units (ICUs), and 6 isolates were collected from the respiratory department
Compared with the total bacterial number, there were no significant differences found between different genders (p = 0 359), while the significant differences were found in different age ranges (p = 0 000), different departments (p = 0 000), and different sources (p = 0 016) (Table 2)
Summary
Acinetobacter baumannii is an aerobic, gram-negative coccobacillus that causes nosocomial human infections, in immune-compromised individuals. The strains were called multiple drug-resistant Acinetobacter baumannii (MDRAB), because they were resistant to at least three of five categories of common antibiotics. MDRAB infections have increased rapidly and have become a significant problem in hospitals, in the intensive care unit [2]. More than 10% of hospital-acquired infections are caused by A. baumannii in the United States, leading to a mortality rate greater than 50% in patients with sepsis or pneumonia [3]. Nanometer-sized silver particles (AgNPs) have long been known to have an antibacterial effect. We have illustrated that decreasing proliferation and increasing apoptosis are very important mechanisms of the AgNP antibacterial effect in standard strains of E. coli [12].
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