Abstract
Objective: We study the biomedical optical properties of the color light and near-infrared fluorescence separated-merged imager. Materials and Methods: The color light and near-infrared fluorescence separated-merged imager can illuminate the visible light and the near-infrared light of [Formula: see text][Formula: see text]nm, receiving the reflected light and [Formula: see text][Formula: see text]nm near-infrared fluorescence, and display the color, fluorescence and merge image. ICG solution of different concentration, including standing time, was allocated to study the best imaging condition in vitro, and the depth of fluorescence penetration was studied with 5% agarose gel; the imaging characteristics of the imager was studied using SD rat; and then the SLNs tracing in 4 cases of penile carcinoma was performed. Results: When the concentration of ICG is 13.11[Formula: see text][Formula: see text]mol/L, the fluorescence intensity and the merge image are the best. The maximum depth of fluorescence imaging is 9[Formula: see text]mm in 5% agarose gel, while the bone has the greatest influence on it. The SLNs tracing shows that the imager can locate the SLNs in vitro, to achieve perioperative navigation during biopsy. Conclusion: There are many factors that affect the imaging effect, but the imaging effect of the imager meets the requirement of vision in a wide range, and can effectively trace the SLNs in perioperative period.
Highlights
In recent years, with the development of near-infrared (NIR) °uorescence navigation technology, this technology has shown great clinical value in tumor localization, lymph nodes and important organ imaging and tumor edge determination.[1]
Many scholars have conrmed that NIR °uorescence imaging using Indocyanine green (ICG) plays an important role in sentinel lymph nodes (SLNs) tracing of various cancer, but the biggest disadvantage of pure NIR imaging only displays °uorescence and the operationeld cannot be seen, so intraoperative guidance cannot be carried out e®ectively
The concentration has obvious in°uence on the gray value and signal-to-noise ratio (SNR), the SNR of ICG solution is greater than 9.8 dB, except for the concentration of 1677.51 mol/L, 838.76 mol/L
Summary
With the development of near-infrared (NIR) °uorescence navigation technology, this technology has shown great clinical value in tumor localization, lymph nodes and important organ imaging and tumor edge determination.[1]. The imaging equipment which can fuse the light and the NIR °uorescence will be the inevitable trend of the near NIR navigation in the future For this reason, our team has developed a new type of color light and NIR separated-merged imager. Research shows that the device can display the visible light image, NIR °uorescence image and visible light and NIR °uorescence fusion image in surgicaleld at the same time. This imaging technique will improve the accuracy of °uorescence tracer in surgery and has been applied to the navigation of SLNs biopsy for penile cancer
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