Biomechanism of chlorogenic acid complex mediated plasma free fatty acid metabolism in rat liver.

Sudeep H.V,Dipak Patel,Venkatakrishna K,Shyamprasad K

doi:10.1186/s12906-016-1258-y

Abstract

BackgroundPlasma free fatty acids (FFA) are involved in blood lipid metabolism as well as many health complications. The present study was conducted to evaluate the potential role of chlorogenic acid complex from green coffee bean (CGA7) on FFA metabolism in high fat diet fed rats.MethodsHyperlipidemia was induced in Wistar rats using high-fat diet. The animals were given CGA7/orlistat concurrently for 42 days. The parameters analysed during the study include plasma and liver total cholesterol (TC), Triglycerides (TG) and FFA. AMPK activation in the liver was analysed through ELISA. The multiple factors involved in AMPK mediated FFA metabolism were analysed using western blotting.ResultsCGA7 (50, 100, 150 mg/kg BW) decreased triglycerides (TG) and FFA levels in plasma and liver. CGA7 administration led to the activation of AMP-activated protein kinase (AMPK) and a subsequent increase in the levels of carnitine palmitoyltransferase 1 (CPT-1). There was a decrease in acetyl-CoA carboxylase (ACC) activity as evident by the increase in its phosphorylation level.ConclusionChlorogenic acids improved the blood lipid metabolism in rats by alleviating the levels of FFA and TG, modulating the multiple factors in liver through AMPK pathway. The study concludes that CGA7 complex can be promoted as an active ingredient in nutrition for obesity management.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1258-y) contains supplementary material, which is available to authorized users.

Highlights

Plasma free fatty acids (FFA) are involved in blood lipid metabolism as well as many health complications
The present study investigates the hypolipidemic effects of chlorogenic acid complex (CGA7), a complex of seven isomers of chlorogenic acid from green coffee beans supplied by Department of Phytochemistry, Vidya Herbs Pvt Ltd., in rats
Antibodies for AMP-activated protein kinase α (AMPKα) and its phosphorylated form, acetyl CoA carboxylase (ACC) and phosphor-acetyl-CoA carboxylase (ACC), carnitine palmitoyltransferase 1 (CPT-1), and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA)

Summary

Introduction

Plasma free fatty acids (FFA) are involved in blood lipid metabolism as well as many health complications. The present study was conducted to evaluate the potential role of chlorogenic acid complex from green coffee bean (CGA7) on FFA metabolism in high fat diet fed rats. Reducing the level of plasma FFA and promoting FFA uptake and Chlorogenic acids (CGA) represent a large family of phenolic compounds formed by the esterification of cinnamic acids with quinic acids. A large body of evidence suggest that CGA exhibits anti-obesity activity by alleviating lipid metabolism in high-fat diet-fed rats [6]. Recent studies showed that CGA stimulated glucose transport via increasing expression of GLUT4 and PPAR-γ transcript. This is due to the activation of AMP activated protein kinase (AMPK). There are no scientific reports explaining the detailed mechanism of action of CGA complex on FFA metabolism

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Conclusion