Biomechanical properties of the collagen, PLLA and HA scaffolds with different temperature and porosity

Abstract

This study aims to investigate the effects of three scaffolds with different porosity on MG-63 cell line in terms of proliferation in vitro conditions. In the present study we developed three collagen based PLLA and HA scaffolds with different porosity. Collagen and poly- l -lactic acid (PLLA), collagen and hydroxyapatite (HA; 30 °C), collagen and hydroxyapatite (HA; 37 °C) scaffolds were produced. Osteoblast-like MG63 cells were cultured on to these porous scaffolds in vitro conditions. Cell adhesion, proliferation were studied on these scaffolds. Alkaline phosphatase (ALP) levels in media were measured and morphological evaluations were assessed. The properties of scaffolds with cells were evaluated with scanning electron microscopy (SEM). The biomechanical tensile tests were performed prior to cell seeding by using the test machine (AG-I 10 kN, Shimadzu, Japanese) with 1 mm/min. The data of force–displacement variation was evaluated with parametric test using SPSS 11.0 for Windows. Scaffolds were compared with Kruskal–Wallis test. The biomechanical tensile tests showed that collagen and hydroxyapatite (HA; 37 °C) scaffolds have maximum force ( p <0.005). The results of cells attachment and proliferation on the scaffold show that based PLLA and HA scaffolds with different porosity have good cell biocompatibility. The results of ALP levels and SEM observations suggested that collagen based PLLA and HA scaffolds might improve osteoblastic growth in vitro and shows potential biomaterial integration for a possible therapeutic application in future for clinical studies. This study suggested that scaffold surface morphology might be an important factor for adhesion and growth of osteoblast cells.