Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture

Yosephine Sri Wulan Manuhara,Alfinda Novi Kristanti,Dannis Yuda Kusuma,Rafika Lailiyatul Kurnia Sari

doi:10.15294/biosaintifika.v9i3.9670

Yosephine Sri Wulan Manuhara, Alfinda Novi Kristanti + Show 2 more

Open Access

https://doi.org/10.15294/biosaintifika.v9i3.9670

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Abstract

Gynura procumbens has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of G. procumbens in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of G. procumbens adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance.

Highlights

Gynura procumbens is one species of Asteracea known as a medicinal plant in Indonesia
Adventitious roots were obtained from leaves, which were grown in MS (Murashige and Skoog) medium supplemented with IBA 5 mg/L, sucrose 30 g/L and agar 8 g/L
Supplementation of sucrose 50 g/L significantly increase the growth of adventitious roots

Summary

INTRODUCTION

Gynura procumbens is one species of Asteracea known as a medicinal plant in Indonesia It is a herb which have fast growth and widely distributed in South East Asia, including Indonesia, Malaysia, and Thailand. People use this plant as a traditional medicine for inflammation diseases, diabetes, cancer, and hypertension. This plant is potential to produce secondary metabolites containing alkaloid, flavonoid, and coumarin. This research was conducted to know the effect of sucrose concentration on biomass production of adventitious roots of G. procumbens in three liquid culture medium: shake flask culture, temporary immersion system, and ballon-type bubble bioreactor. Results of this research can be used as the basic method to scale up the production of biomass from G. procumbens adventitious roots

METHODS

RESULTS AND DISCUSSION

CONCLUSION