Abstract
The objective of the present study was to evaluate the growth kinetics of Aphanothece microscopica Nägeli under different conditions of temperature, light intensity and CO 2 concentration. The growth kinetics of the microorganism and carbon biofixation were evaluated using a central composite design, considering five different temperature levels (21.5, 25, 30, 35 and 38.5 °C), light intensities (0.96, 3, 6, 9 and 11 klux) and carbon dioxide concentrations (3, 15, 25, 50 and 62%). The results obtained showed the effects of temperature, light intensity and CO 2 concentration ( p < 0.05) on the photosynthetic metabolism of the microorganism. Response surface methodology was adequate for process optimisation, providing a carbon fixation rate to the order of 109.2 mg L −1 h −1 under conditions of 11 klux, 35 °C and 15% carbon dioxide, representing an increase of 58.1% as compared to the conditions tested initially.
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