Abstract
Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp. ICE-L grown in NH4Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH4NO3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids (PUFAs) production, NH4NO3 and NH4Cl media performed better than nitrogen-deficiency and KNO3 media. Furthermore, NH4NO3 and NH4Cl media elucidated better results on C18:3 and C20:5 productions while KNO3 and -N conditions were better in C16:0, C18:1 and C18:2, comparatively.
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