Abstract
This study aims to determine the type of metabolites contained in the extraction of Ipomea carnea canopy biomass and its inhibitory effect in vitro against Trichoderma asperellum Tc-Jjr-02 which has been tested as an active ingredient in bio fungicides and biofertilizers. The initial stage of the research is the determination of the metabolites contained in the extract with methanol solvent through Gas Chromatography-Mass Spectrophotometry (GCMS) analysis. The next step was an in vitro test of the extract's inhibitory power to the growth of T. asperellum fungal colonies during the incubation period of up to 96 hours. The results of GCMS analysis of extracts with methanol solvents obtained 24 types of metabolites, some of which can inhibit fungal growth. Crude extract of canopy biomass of I. carnea at a concentration of 2% inhibited the growth of all Trichoderma isolates by 20.97±3.36% (Tc-Jjr-02), 38.15±32.59% (Tc-Sba-01), and 45.30±1.86% (Tc-Jro-01), while at a concentration of 20% inhibited isolates Tc-Sba-01 and Tc-Jro-01 were 35.19±4.90% and 42.59±1.85% in vitro at 96 hours after inoculation, respectively. The isolate of T. esperellum Tc-Jjr-02 has the potential to be formulated with I. carnea biomass as compost in the future.
Highlights
Each country develops a national scenario to reduce CO2 emissions according to the Paris Agreement [1], including in the agricultural sector
This study aims to determine the type of metabolites contained in the extraction of Ipomea carnea canopy biomass and its inhibitory effect in vitro against Trichoderma asperellum Tc-Jjr-02 which has been tested as an active ingredient in bio fungicides and biofertilizers
Several caryophyllene analogs have been found with various biological functions including cytotoxic characteristics and antimicrobial activity [15], and are more effective against gram-positive bacteria than gram-negative bacteria [16]. (4H)-Benzofuranone, 5,6,7,7a-tetrahydro-6-hydroxy-4,4,7atrimethyl, (6S-cis)- known as loliolide [17] is an identical compound with the allelochemical 2,4 -dihydroxy-7-methoxy-1,4-benzoxazine-3-one (DIMBOA) which acts as an allelopathic which can inhibit the growth of root cells [18]; This compound is a potent antioxidant isolated from Sargassum horneri which has a positive effect on cell growth [19]
Summary
Each country develops a national scenario to reduce CO2 emissions according to the Paris Agreement [1], including in the agricultural sector. The facts show that the application of synthetic chemical pesticides results in the suppression of non-target organisms [2], the resistance of nuisance organisms [3], environmental pollution [4], and threatens human health [5]. For this reason, efforts to find and use environmentally friendly alternative materials in agricultural cultivation must continue to be improved. Research and application of Trichoderma fungi have been developed to increase plant resistance and protect plants from attack by harmful pathogens. Its potential as a biopesticide that is applied by smearing the stems of citrus plants provides prospects for application to various other agricultural crops
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