Biomass derived nitrogen functionalized carbon nanodots for nanomolar determination of levofloxacin in pharmaceutical and water samples.

Abstract

Binder-free and efficient electrochemical sensing of levofloxacin (LF) was successfully developed based on the nitrogen-doped carbon nanodots (NCNDs). The NCNDs were synthesized by hydrothermal carbonation (180°C for 12 h), and the heteroatom was embedded in aqueous solution of ammonia (NH3). Spectral and microscopic characteristization techniques were used to analyze the topological, crystallinity, and chemical binding behavior of synthesized biomass functional material. HR-TEM image revealed a uniform spherical dot (2.96 nm), and superior quantum yield efficiency (0.42 Φ). The NCNDs was drop coated on a glassy carbon electrode (GCE) and electrochemical sensing of LF was performedby cyclic voltammetry (CV), differential pulse voltammetry (DPV), and amperometric i-t curve in phosphate-buffered saline (PBS; pH = 7.0). The NCNDs modified electrode showed a sharp oxidation peak at +0.95 V (vs. Ag/AgCl) with a four-fold higher current response than the bareGC electrode. The NCNDs/GCE surface not only increases the current response, but has lower detection potential, and facilitates electron transfer reaction. Under optimized working parameters, the NCNDs/GCE showed wide linear concentrations rangefrom 200 nM to 2.8 mM and a low detection limit (LOD) of 48.26 nM (S/N = 3). The electrode modified with NCNDs has high electrochemical sensing stability (RSD = 1.284 ± 0.05% over 5 days), and superior reproducibility (RSD = 1.682 ± 0.06% (n = 3)). Finally, theNCNDs modified GC electrode was successfully applied to quantify the concentration of LF in drug and river water samples with acceptable recovery percentages of 96.60-99.20% and 97.20-99.00% (n=3), respectively.