Abstract
Radiation technology is widely used to produce changes in biosystems. The goal of this work is to determine the variation induced in male parents and F1 Ceratitiis capitata when gamma-irradiated as pupae using substerilising doses (50 and 70 Gy and sterilising dose 90 Gy. Biological studies showed that the percent adult emergence of irradiated pupae decreased as the dose increased and also the percent egg hatchability decreased as the dose increased, also percent pupation and percent emergence of F1 decreased as the dose increased. Comparing DNA (using RAPD-PCR) of untreated adults, adults irradiated with sterilising dose of 90 Gy and parents irradiated with substerile dose (50 and 70 Gy) and their resultant F1. The results revealed that some extra bands appeared and others disappeared, as a result of irradiation. The appearance of extra bands may be due to the repair mechanism of the irradiation damaged DNA. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that irradiation of parents with 50 and 70 Gy and their F1 are different from the unirradiated pupae It was concluded that the sterile male technique could be used as a benefit tool in controlling Ceratitiis capitata.
Highlights
The Mediterranean fruit fly, Ceratitis capitata is considered a major pest of fruit production worldwide
Different letter means statistically significant at P < 0.05 (Tukey Pairwise Comparisons test)
Our results show that the exposure of the pupae to gamma radiation caused a reduction in egg hatchability and an increase in % sterility as the dose increase
Summary
The Mediterranean fruit fly, Ceratitis capitata is considered a major pest of fruit production worldwide. It is a highly polyphagous species with the capability to adapt and spread into several different environments, giving it the status of a threating invasive species for fruit production in many countries (Figueroa 2018). Management of Ceratitis capitata using traditional chemicals have limited success, and many alternative approaches have been developed and evaluated, including the use of radiation to induce dominant lethal mutations in the sterile insect technique (SIT), it is a major component of many large and successful programmes for pest suppression and eradication. Substitutional, eco-friendly methods to control insect pests include the SIT, or the partial male sterility technique ( called inherited sterility IS or F1 sterility). In SIT and Fl sterility, mass production and release of large numbers of genetically altered sterile males are released into the treated area in quantities that surpass the estimated population size of wild
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