Biomarkers as predictive tools to test the in vivo anti-sarcoptic mange activity of propolis in naturally infested rabbits.

Dina M Metwally,Reem A Alshalhoop,Shatha A Eisa,Ebtesam M Al-Olayan

doi:10.1042/bsr20180874

Dina M Metwally, Reem A Alshalhoop + Show 2 more

Open Access

https://doi.org/10.1042/bsr20180874

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Journal: Bioscience reports	Publication Date: Dec 7, 2018
Citations: 3	License type: CC BY 4.0

Affiliation: Zagazig University, King Saud University

Abstract

The present study was designed to investigate the use of specific biomarkers, such as albumin, serum total protein, aspartate amino transferase (AST), globulin, alanine amino transferase (ALT), serum cortisol and alkaline phosphatase (ALP), as predictive tools for sarcoptic mange in rabbits. A total of 40 naturally infested rabbits were equally divided into four groups.Thirty infested rabbits were administered with three different treatments (propolis,ivermectin, and propolis with ivermectin) and were compared to10 infested un-treated rabbits. The impact of treatment was assessed via microscopic examination of skin scrapings, clinical signs, and blood measurements relating to the liver. The present study demonstrated that topical application of 10% propolis ointment resulted in complete recovery from clinical signs and complete absence of mites based on microscopic examination after 10–15 days of treatment. Moreover, AST, ALP, ALT, and cortisol were determined to be acceptable biomarkers to track the response of diseased rabbits to the therapeutic use of propolis.

Highlights

Mange triggered by Sarcoptes scabiei var. cuniculi remains a common occurrence among pet rabbits
Since the introduction of ivermectin for the treatment of scabies, reports of adverse events have been rare this drug can cause cardiac dysfunction and hepatitis [29]
The total protein level of the group that was treated with ivermectin was significantly high

Summary

Introduction

Mange triggered by Sarcoptes scabiei var. cuniculi remains a common occurrence among pet rabbits. Cuniculi remains a common occurrence among pet rabbits. This parasite is unique in that it is able to inhabit the epidermal layer of the skin where the larvae’s feeding behavior and nymphs lead to hypersensitivity reactions, hyperkeratosis, inflammation, alopecia and seborrhea [1,2]. Propolis containing products have been marketed for human use for different purposes [16], and researchers have been involved in the study of isolated compounds responsible for propolis’ therapeutic actions. The current investigation evaluated the c 2018 The Author(s)

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