Biomarker responses in perch (Perca fluviatilis) under multiple stress: Parasite co-infection and multicomponent metal mixture exposure

Abstract

Parasitic infections may cause damage to the host immune system (i.e. fish), thereby endangering its health and weakening its responses to other types of stressors. Therefore, exposure to different kinds of natural or anthropogenic stressors can lead to unexpected toxicity outcomes in aquatic organisms. This study examined the haematological, genotoxic and cytotoxic effects of the co-infection with the protozoan parasite (Trichodina sp.) and the pathogenic oomycete (Saprolegnia parasitica) in Perca fluviatilis alone and in combination with chemical stress (environmentally-relevant aqueous concentrations of metal mixtures). Haematological analyses such as red cell and white cell indices revealed that chemical and biological stressors, used singly and in combination, exerted adverse effects on fish health. Changes in haematological indices induced by exposure to each of the above-mentioned stressors separately and by combined exposure to all of them suggested the multiple stress-induced inflammation process in the exposed fish. The cytogenetic damage inflicted by the S. parasitica and Trichodina sp. co-infection and multiple stress was revealed in fish erythrocytes. This information is expected to contribute to the elucidation of how multiple stressors impact on responses of haematic indices, geno- and cytotoxicity endpoints in P. fluviatilis. Assessment of the risk associated with multiple stressors is expected to prove valuable for the effective aquatic environment management (Løkke et al., 2013 and references therein).