Abstract
ProteinChip Array technology has been used to identify unique biomarker signatures in patients with conditions such as cancer and acute coronary syndrome. This technique employs Surface Enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry, allowing for proteomic profiling over a broad molecular weight range. To test the hypothesis that aging processes generate unique biomarker profiles, citrated plasma samples from 9, 18, and 26 month-old, male and female F344 rats were subjected to ProteinChip Array analysis. Utilizing Strong Anion Exchange (SAX-2) ProteinChips, the biomarker profile was analyzed in the 0-150kDa range. Targeted analysis was performed in the lower molecular weight range of 3-9kDa. Specific biomarkers at 6.9, 8.2, 8.7, and 8.8kDa were up-regulated in females at 18 as compared to 9 months of age, whereas these changes were not obvious in the male group. At 26 months, these biomarkers tended to return toward 9-month levels in females, whereas an upward trend was observed in male rats. These data suggest that age-associated changes in the serum biomarker profile differ in male and female rats. Furthermore, the differential biomarker profile in the lower molecular weight range (<10 kDa) suggests that proteases may play a role in observed age- and gender-associated changes.
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