Biomarker in human breast cancer cells

Abstract

Multiplex measurement of biomarkers in human breast cancer cell culture supernatesJane Schmidt, Ph.D, Michael Hall, Richard Fuerstenberg. R&D Systems, Inc. Minneapolis, Minn.The mixture of biomarkers that is produced in the tumor microenvironment has an important role in cancer pathogenesis. For example, cytokines that are released in response to infection, inflammation and immunity can function to either promote or inhibit tumor development and progression. The multiplex immunoassay has become an efficient tool both for rapid screening and more in‐depth analysis of cancer cell supernates, while requiring only a single, small sample volume. In this study, the VersaMAP™ Development System was used to rapidly and simultaneously screen for human biomarkers in supernates from six human breast cancer cell lines. Fifteen positive biomarkers were identified and biomarker profiles were established for each of the cell lines. The VersaMAP Development System, developed by R&D Systems,Inc., is a customized assay system designed for the simultaneous measurement of multiple biomarkers from a single 50 μL sample. It offers the ability to measure up to 10 biomarkers chosen by the user from a continuously expanding library of 39 available analytes. The assay is available in a custom premixed form, giving the user total flexibility to choose their multiplex panel. The assay system is a bead‐based multiplex immunoassay designed for use with a Luminex® or Bio‐Rad® Bio‐Plex® analyzer.